Fungi Transformation Methodologies and Cloning of Sago Palm chitinase 2 cDNA

Nur Maisarah Afiqah, Arizal (2022) Fungi Transformation Methodologies and Cloning of Sago Palm chitinase 2 cDNA. [Final Year Project Report] (Unpublished)

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Abstract

The sago palm (Metroxylon sagu) has become one of the most prevalence source of starch especially in the region of Sarawak, Malaysia. However, fungal infection has become a prevalent problem due to the intensive cultivation in M.sagu and crop plants. Fungal infections will eventually lead to wilting of roots which causes crop organs to be damaged and biotic stress in the crop plants will be stimulated. From M.sagu, the enzyme chitinase can be extracted where the chitinase are able to breakdown the chitin structure in that made up the wall of fungus. In this research specifically, the chitinase have already been isolated and this research will focus on the cloning of chitinase and genetic transformation in Fungi. The previously cloned samples were revived in both LB Broth and LB Agar with added chloramphenicol, CAM for expression in fungi. Colony PCR were performed to ensure the right colony was revived containing pGSA + chi2. Fungi was chosen for the genetic transformation of chi2 as the plasmid carrying chi2 has a larger size (9.3 kb).

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