Utilization of Leaf Litter from Filicium decipiens (Japanese Fern Tree) for Laccase Enzyme Production by Marasmius cladophyllus UMAS MS8

Nur Farah Nadirah, Jefferia (2022) Utilization of Leaf Litter from Filicium decipiens (Japanese Fern Tree) for Laccase Enzyme Production by Marasmius cladophyllus UMAS MS8. [Final Year Project Report] (Unpublished)

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Abstract

Marasmius cladophyllus has the ability to produce laccase for the degradation of lignin. Lignocellulosic residues used as substrate are important lignin sources and become the potential inducer for laccase production. To date, limited study on the ability of leaf litter from F. decipiens to be used as substrate for fermentative enzyme production have prompted the investigation on the ability of M. cladophyllus to produce laccase using F. decipiens as a substrate through both solid-substrate fermentation (SSF) and submerged fermentation (SmF). Submerged fermentation resulted in the highest laccase activity throughout the 12-days fermentation period, with maximum activity attained at 0.508 U/g on the twelfth day of fermentation. However, for solid-substrate fermentation, the highest average reading was 0.086 U/g but there was no laccase activity present as no colour change in the assay was observed. The findings obtained in this study confirmed the potential approach using cheap lignocellulosic waste, which is leaf litter of F. decipiens for laccase production by M. cladophyllus through submerged fermentation.

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