MICROBIAL AND PHYSIOCHEMICAL ANALYSIS OF WATER FROM SEMENGGOK INLAND FISHERY CENTRE, KUCHING, SARAWAK

LOKE, XIN YA (2022) MICROBIAL AND PHYSIOCHEMICAL ANALYSIS OF WATER FROM SEMENGGOK INLAND FISHERY CENTRE, KUCHING, SARAWAK. [Final Year Project Report] (Unpublished)

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Abstract

Water quality is emphasized by the aquaculture sector as it affects the health and growth performance of aquaculture species. However, due to the outbreak of COVID-19 pandemic in Malaysia, various sectors including aquaculture sector, have been instructed to shut down as a lockdown alternative to control the spread of the disease. The total lockdown has made impossible to give proper management to the aquaculture ponds and indirectly led to the high mortality of aquaculture organisms. High mortality has led to rapid microbial growth, which affected the water quality and the balance in the aquaculture system. In this study, the characteristics of aquaculture environmental samples were determined. Water and soil samples were collected from three randomly selected ponds in the Semenggok Inland Fishery Centre, Kuching, Sarawak and the physiochemical and biological parameters were analysed. Bacteria were isolated from both water and soil samples and then characterised. Boiling-centrifugation method was used for DNA extraction of the bacteria. (GTG)5-PCR was utilized to screen for clonal diversity among the isolates. A dendrogram was constructed using GelJ 1.0 software from the banding profile of (GTG)5-PCR products. Out of all the isolates analysed, 11 representative isolates were selected for 16S rRNA sequencing based on the grouping from the dendrogram. The 11 isolates were identified as Brevundimonas sp., Staphylococcus sp., Pseudomonas sp., Escherichia sp., Ralstonia sp., and Exiguobacterium sp.. The isolates were tested for antibiotic resistance using the disc diffusion method. Most of the isolates tested were resistant to ampicillin (10 μg), penicillin (10 μg), and streptomycin (10 μg). The MAR index of isolates were calculated, ranged from 0.143 to 0.714, indicating high possibility of culturing fish in the contaminated water. This study revealed the risk of presence of multiple antibiotic resistance (MAR) bacteria in the fishery centre. Therefore, the fishery centre should improve the aquaculture system by constantly monitoring and also provide a proper management to the wastewater to minimise the distribution of MAR bacteria.

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