Leong, Yee Wei. (2006) Development of single chain variable fragment antibodies against dengue virus. Masters thesis, Institute of Health andCommunity Medicine.
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Development of single chain variable fragment antibodies against dengue virus-cloning, expression and functional analysis (fulltext).pdf Restricted to Registered users only Download (14MB) |
Abstract
The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | Thesis (M.Sc.) -- Universiti Malaysia Sarawak, 2006. |
| Uncontrolled Keywords: | Immunoglobulins, Biotechnology, Immunotechnology, Laboratory manuals, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Postgraduate, research, Universiti Malaysia Sarawak |
| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine Faculties, Institutes, Centres > Institute of Health and Community Medicine |
| Depositing User: | Saman |
| Date Deposited: | 29 Jul 2016 07:34 |
| Last Modified: | 07 Mar 2023 08:40 |
| URI: | http://ir.unimas.my/id/eprint/12719 |
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