Development of single chain variable fragment antibodies against dengue virus

Leong, Yee Wei. (2006) Development of single chain variable fragment antibodies against dengue virus. Masters thesis, Institute of Health andCommunity Medicine.

[img]
Preview
PDF
Development of single chain variable fragment antibodies against dengue virus-cloning, expression and functional analysis (24 pages).pdf

Download (1MB) | Preview
[img] PDF (Please get the password from Digital Collection Development Unit, ext : 3932 / 3914)
Development of single chain variable fragment antibodies against dengue virus-cloning, expression and functional analysis (fulltext).pdf
Restricted to Registered users only

Download (14MB)

Abstract

The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens.

Item Type: Thesis (Masters)
Additional Information: Thesis (M.Sc.) -- Universiti Malaysia Sarawak, 2006.
Uncontrolled Keywords: Immunoglobulins, Biotechnology, Immunotechnology, Laboratory manuals, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Postgraduate, research, Universiti Malaysia Sarawak
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine
Depositing User: Saman
Date Deposited: 29 Jul 2016 07:34
Last Modified: 29 Jul 2016 07:35
URI: http://ir.unimas.my/id/eprint/12719

Actions (For repository members only: login required)

View Item View Item