Isolation of antibiotic resistance among pseudomonas aeruginosa from aquaculture environment and rivers in Sarawak

Soh Khar, Mun (2015) Isolation of antibiotic resistance among pseudomonas aeruginosa from aquaculture environment and rivers in Sarawak. [Final Year Project Report] (Unpublished)

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P eudomonas aeruginosa, a Gram-negative bacillus-shaped bacterium can be found in various environments. The high adaptability of P. aeruginosa is devoted by the simple nutritional requirement and thus contributed P. aeruginosa as an opportunistic pathogen. In this study, P. aeruginosa was isolated from water and fish in rivers and aquaculture farm in Sarawak. A total of thirty-eight isolates were successfully isolated using CHROMagarâ„¢ P eudomonas. The isolates were SUbjected to a series of biochemical tests such as gram staining, MacConkey agar test, oxidase test, triple sugar iron agar test, citrate test, catalase test, starch hydrolysis test, and nitrate reduction test. Based on the biochemical tests, two isolates possess the characteristics of P. aeruginosa. These isolates were further confirmed through molecular identification using 16S rRNA PCR analysis and DNA sequencing. These two P. aeruginosa were screened against nine antibiotics: piperacillin, ceftazidime, imipenem, meropenem, gentamicin, tobramycin, amikacin, ciprofloxacin, and norfloxacin using the disk diffusion assay. Both isolates were susceptible to all the antimicrobial agents tested. This suggested that all these antimicrobial agents are effective against P. aeruginosa.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Pseudomonas aeruginosa, aqoaculture, biochemical test, 16S rRNA gene sequencing, antibiotic resistance, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 21 Mar 2016 06:44
Last Modified: 06 Aug 2021 16:28

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