Isolation of antibiotic resistance among pseudomonas aeruginosa from aquaculture environment and rivers in Sarawak

Soh Khar, Mun (2015) Isolation of antibiotic resistance among pseudomonas aeruginosa from aquaculture environment and rivers in Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

P eudomonas aeruginosa, a Gram-negative bacillus-shaped bacterium can be found in various environments. The high adaptability of P. aeruginosa is devoted by the simple nutritional requirement and thus contributed P. aeruginosa as an opportunistic pathogen. In this study, P. aeruginosa was isolated from water and fish in rivers and aquaculture farm in Sarawak. A total of thirty-eight isolates were successfully isolated using CHROMagar™ P eudomonas. The isolates were SUbjected to a series of biochemical tests such as gram staining, MacConkey agar test, oxidase test, triple sugar iron agar test, citrate test, catalase test, starch hydrolysis test, and nitrate reduction test. Based on the biochemical tests, two isolates possess the characteristics of P. aeruginosa. These isolates were further confirmed through molecular identification using 16S rRNA PCR analysis and DNA sequencing. These two P. aeruginosa were screened against nine antibiotics: piperacillin, ceftazidime, imipenem, meropenem, gentamicin, tobramycin, amikacin, ciprofloxacin, and norfloxacin using the disk diffusion assay. Both isolates were susceptible to all the antimicrobial agents tested. This suggested that all these antimicrobial agents are effective against P. aeruginosa.

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