Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)

Siti Izyan Liyana, Kamarol (2015) Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu). Masters thesis, Universiti Malaysia Sarawak, (UNIMAS).

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Abstract

Sago palm (Metroxylon sagu Rottb.) is an economic valuable crop and cultivated widely in Sarawak. The potential use as starch resource for the food industry required an increase its production. Under certain conditions, some sago palms do not develop well and become stunted. This is considered as non-trunking sago palm. Morphologically, this type of sago palm is not normal and this led to wastage of various resources and affects the starch production. The research objective was to understand the molecular process that affect non-trunking and starch synthesis in sago palm. Representational Difference Analysis was performed to identify the differences between trunking and non-trunking sago palm transcriptome. This subtractive hybridization technique detects the differences in gene expression. After three rounds of hybridization, the sequences obtained were compared with the sequences in NCBI database. Results showed the activated genes in response to the non trunking sago palm have similarity to genes that are implicated in plant growth, cell metabolism and salt or osmotic stress. The differential expressed genes in trunking sago palm code for genes related to growth development and signalling in neutralizing the imbalance in plant stress. The information obtained through this work can be used to develop database related to trunking and non-trunking sago palm.

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