Henry Sum, Magdline Sia and Andrew, Anna and Aren Maling, Milda (2015) Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System. Journal of biochemistry, microbiology and biotechnology, 3 (1). pp. 26-29. ISSN 2289-5779
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Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System (abstract0.pdf Download (218kB) | Preview |
Abstract
Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The recombinant E1 protein with N-term 6-His residues protein was successfully expressed and purified as confirmed by SDS-PAGE and western blot analysis. The seroreactivity of the recombinant protein was evaluated in immunoassay for anti-CHIKV IgM and IgG antibodies. The recombinant antigen showed 69% sensitivity and 100% specificity for anti-CHIKV IgG by dot blot assay. Detection of anti-CHIKV IgM by dot assay showed 79% sensitivity and 100% specificity. No cross reactivity of the antigen was observed with anti-dengue virus serum samples. The results strongly support that the recombinant E1 protein has potential to be used as diagnostic antigen. The used of the antigen in a dot blot assay gives an advantage for laboratory detection without the need of any specialised equipment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chikungunya virus, E1 glycoprotein, baculovirus system, anti-CHIKV IgM, immunoassay, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology Q Science > QR Microbiology > QR355 Virology R Medicine > R Medicine (General) |
| Divisions: | Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine Faculties, Institutes, Centres > Institute of Health and Community Medicine |
| Depositing User: | Ramji |
| Date Deposited: | 03 Nov 2015 07:23 |
| Last Modified: | 03 Nov 2015 07:23 |
| URI: | http://ir.unimas.my/id/eprint/9288 |
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