Occurrence and quantification of vibrio parahaemolyticus in shrimp aquaculture and environment

Nur Bainun, Binti Mohd Zain (2013) Occurrence and quantification of vibrio parahaemolyticus in shrimp aquaculture and environment. [Final Year Project Report] (Unpublished)

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Abstract

Vibrio spp. occurs naturally in aquatic environments and are one of the most commonlyoccuring bacteria in shrimp farming. At least 14 species had been found in panaeid shrimp culture system. V. parahaemolyticus can cause outbreak of gastroenteritis due to the consumption of raw or undercooked seafood. V. parahaemolyticus in the aquaculture may cause vibriosis to shrimp. This project was conducted using water and shrimp samples taken from a shrimp aquaculture in Bako, Sarawak to study the presence of toxR gene of V. parahaemolyticus by utilizing species specific PCR and enumeration with MPN method. Statistic analyses were carried out to identify the relationship and correlation between the occurrence of V. parahaemolyticus and environmental parameters comprises of pairedsample t test and Pearson correlation analysis. The findings indicated 86% water samples and 14% shrimp samples harboured toxR gene for V. parahaemolyticus. There were no significant difference in both statistical analyses. Hence, the present study highlighted the potential risk of V. parahaemolyticus from shrimp aquaculture and environment to infect human and animal upon consumption.

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