Isolation of heterocyclic hydrocarbon from mangrove enviroment

Nur Syahirah, Binti Othman (2013) Isolation of heterocyclic hydrocarbon from mangrove enviroment. [Final Year Project Report] (Unpublished)

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Abstract

Heterocyclic hydrocarbon contamination is a serious issue occurring throughout the world. To-date, only a few studies have been conducted on the heterocyclic hydrocarbon-degrading bacteria from mangrove environment. The soil sample was taken from mangrove environment at Asajaya, Sarawak. In this research, Dibenzofurans (DBF)-degrading bacteria, SI, Dibenzothiophene (DBT)-degrading bacteria, S2, and Fluorene-degrading bacteria, S3, was detected and isolated from the mangrove soil sample. ONR7a media and marine broth and agar were used for culture emichment, microorganism screening and isolation of the microbes. Polymerase Chain Reaction (PCR) was used for the detection of the hydrocarbon-degrading bacteria at molecular level. Physiological and biochemical tests were also performed for detection and characterization of the bacteria. All the bacteria were Gram positive bacteria, positive catalase test, negative oxidase test and negative production of indole. SI bacteria were motile and able to reduce sulphur into hydrogen sulphide whereas S2 and S3 bacteria were non-motile and not able to reduce SUlphur into hydrogen sulphide.

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