Water quality and characteristics of bacteria from fish facility

Kong, Chak Yien (2012) Water quality and characteristics of bacteria from fish facility. [Final Year Project Report] (Unpublished)

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Abstract

Maintaining the quality and safety of the water is important as the contamination of the water can cause serious illness or even death among the creatures in the water. In this study, chemical and microbiological water quality was determined. Samples of water were collected from fish facility of Department of Molecular Biology, Faculty of Resource Science and Technology, UNIMAS. These water samples were analysed for the presence of bacteria. Bacteria were isolated from the water and were characterized. The DNA extraction of the bacterial isolates was performed by boiling-centrifugation method. The genetic variations among the isolates were analysed by (GTG)5 PCR. (GTG)5 PCR analysis indicated that the isolates were grouped into 2 main clusters. Then, the isolates were picked from the dendrogram for PCR to amplify the 16S rRNA gene of the isolates. Twelve isolates were chosen for sequencing analysis. Among the twelve isolates, eight isolates were Aeromonas veronii, two isolates are Aeromonas jandaei, and the last two isolates are Plesiomonas shigelloides and Pseudomonas alcaligenes respectively. All isolates from the water were tested for their antibiotic resistance by disc diffusion method. AU of the isolates were tested to be resistant towards ampicillin. penicillin and gentamicin. The MAR index of the isolates ranged from 0.455 to 0.727. The multiple antibiotic resistances of the isolates indicate the high potential hazard associated with antibioticresistant bacteria in water.

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