Screening of Grb10 interacting GYF domain protein 1b (GIGYF 1b) gene in zebrafish (danio rerio) development

Pang, Shek Li (2012) Screening of Grb10 interacting GYF domain protein 1b (GIGYF 1b) gene in zebrafish (danio rerio) development. [Final Year Project Report] (Unpublished)

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Abstract

GrblO Interacting GYF domain (GIGYF 1) and Grb 10 interacting GYF domain 2 belong to GIGYF family genes. These two genes interact with Grb I 0, an adapter protein activate Insulin-like Growth Factor-I (lGF-I) through two-hybrid screening. Function of these two genes is regulating tyrosine kinase receptor signaling including IGF-I and insulin signaling. Recently, GIGYF 2 has been nominated as candidate gene involved in Parkinson's disease (PO) as it fallen within PARK II locus, thus, GIGYF 1 was believed it is related in PO since GIGYF 1 is homologous with GIGYF 2. However, functions of GIGYF 1 are remain largely unknown, thus this study might give a hint of function of GIGYF 1. Zebrafish is the organism that suitable for human disease study because zebrafish will often have two GIGYF 1 genes that are GIGYF la and GIGYF I b which are both orthologs of a single mammalian gene. The GIGYF I has an extra tetraploidization with the occurrence of genome duplication. Duplication-Degenerative-Complementation event enable further study of GIGYF I gene, including its functional role in PD. GIGYF I b gene was cloned into pGEM®-T Easy vector and the plasmid with the inserted gene was extracted and stored and ready for probe synthesis for in situ hybridization.

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