Screening and characterization of fibrolytic bacteria from sago waste

Mohd Almuhsen, Karim (2012) Screening and characterization of fibrolytic bacteria from sago waste. [Final Year Project Report] (Unpublished)

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Abstract

A total of 16 bacteria were successfully isolated from the sago waste after selectively enriched in media containing 1 % (w/v) of corn cobs. Screening of the bacterial isolates was done by using Congo-red staining method after the bacterial strains was cultured onto the CMC agar plates and 1 % corn cobs agar plates, respectively. Four isolates were chosen and selected for further analysis due to their ability to produce large halo and characterized. Standard biochemical test, morphological characterization, cellulase enzyme assay, xylanase enzyme assay and molecular characterization were done on these four isolates. Enzyme assays were conducted using ONS method. After ONA sequencing with 16s rONA analysis, the isolates were found to be similar to Bacillus pumilus and Bacillus subtiUs.

Item Type:	Final Year Project Report
Additional Information:	Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords:	Starch,Cornstarch, fibrolytic bacteria, biochemical test, sago waste, screening, characterizing, enzyme, 16s rONA, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate
Subjects:	Q Science > Q Science (General)
Divisions:	Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User:	Karen Kornalius
Date Deposited:	02 Jul 2015 00:55
Last Modified:	28 Aug 2023 04:15
URI:	http://ir.unimas.my/id/eprint/8222

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