Trial Biodeinking of Mixed Office Paper Using the Crude Enzymes Produced By Bacillus amyloliquefaciens Umas 1002 and Bacillus licheniformis P1

Shahirah, Che Amat (2010) Trial Biodeinking of Mixed Office Paper Using the Crude Enzymes Produced By Bacillus amyloliquefaciens Umas 1002 and Bacillus licheniformis P1. [Final Year Project Report] (Unpublished)

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Abstract

Amylase and cellulase production from Bacillus licheniformis P1 and Bacillus amyloliquefaciens UMAS 1002 was enhanced using Submerged Liquid Fermentation (SmF) with respect to the use of types of substrates, time course of incubation and volume of inoculums. Banana peel was chosen as the best substrate for both enzymes production. An optimum production of amylase and cellulase was achieved after 12 h time course of incubation. Besides, both enzymes were highly produced with the use of 3 % (v/v) inoculums percentage. A further study was continued by performing the enzymatic deinking trials. The crude enzymes used were those extracted from the previous experiment which was already optimized. As comparison to the enzymatic deinking, a conventional chemical deinking was set up, plus a set of control assay. The deinking trials were quite successful with some improvement and modifications must be done for future research. Although enzymatic deinking pulp showed lower level of brightness compared to the chemical treatment, the detection of black spots was much more reduced.

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