Isolation and Identification of Escherichia coli from Raw Vegetables, in Kuching, Sarawak

Chua, Jasmine Hsien Ying (2010) Isolation and Identification of Escherichia coli from Raw Vegetables, in Kuching, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Escherichia coli is a common bacteria that can be found in human intestine and is considered harmless until one of the strain had been identified to cause an outbreak of bloody diarrhea. Besides through direct contamination with infected feces, eating raw food might also be a source of transmission. In this study E. coli was isolated from raw vegetables that were bought from three different markets in Kuching. The three main vegetables were choy sum, lettuce and kai-lan. Buffered Peptone Water (BPW) and E. coli broth were used as enrichment media while Eosin-Methylene Blue agar was used for isolation. Additionally, Sorbitol MacConkey Agar was used to isolate E. coli O157:H7. Through series of biochemical test 13 isolates were identified as E. coli while only 2 samples were positive for the presence of E. coli O157:H7.

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