Profiling of microbes with plant-growth promoting traits from sago humus

Ng, Ping Ping (2009) Profiling of microbes with plant-growth promoting traits from sago humus. [Final Year Project Report] (Unpublished)

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Abstract

Sago hampas undergoes decompositions by microorganisms to become stable and simpler form organic compounds called sago humus. In this study, screening tests for plant-growth promoting traits were done on all the isolated bacteria from sago humus collected at Mukah. For bacteria that show positive result were further identified by using biochemical test, morphology and molecular methods. From the 43 isolates that had successfully isolated from 5 sago samples, 8 isolates demonstrated positive results for at least one of the 4 screening tests. Isolate 3 and 4 had ability to produce indole-acetic acid (IAA) and ammonia. Isolate 6 was able to produce hydrogen cyanide (HCN) and ammonia. Other 5 isolates showed only the ability in production of ammonia. All the 8 isolates showed positive result for catalase test and there were 3 isolates demonstrated positive result for oxidase test. Two of the isolates were capable to use citrate as sole source of carbon. Most of the morphologies of the bacteria were in rod shape and belong to gram-negative. Bacillus subtilis and Peseudomonas as PGPB had successfully been identified. In this study, molecular method was considered as direct method in identifying bacteria.

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