Genotyping escherichia coli in animal faeces using pulsed field gel electrophoresis (PFGE)

Sarina, Niyup (2008) Genotyping escherichia coli in animal faeces using pulsed field gel electrophoresis (PFGE). [Final Year Project Report] (Unpublished)

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Abstract

Escherichia coli is one of a group of bacteria that is predominantly present in the intestines and faeces of warmblooded mammals, including wildlife, livestock, and humans. Pulsed field gel electrophoresis had been applied widely to study the genetic diversity of E. coli. In this study, genetic variations of 25 E. coli isolates from different animals; cattle, chicken, and swine, as commercial livestock was examined by pulsed field gel electrophoresis digested with the restriction enzyme, Xba1. PFGE result that was analyzed using Dice Correlation and UPGMA methods revealed the highest similarities of E. coli between cattle and chicken, which is 64.38%. The highest similarities of E. coli within animals, was found within chicken faeces, which is 70.37%. PFGE results successfully categorised E. coli to their sources and showed a close genetic relationship (53.64% to 70.37%) between E. coli strains belonging to the same animals. The result obtained showed that PFGE is a powerful tool to explore the genetic relationship among E. coli isolates in animal faeces.

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