Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer

Suliana, Charles Nyucong (2008) Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer. [Final Year Project Report] (Unpublished)

PDF (Please get the password by email to repository@unimas.my , or call ext: 3914 / 3942 / 3933) Suliana(fulltext).pdf Restricted to Registered users only Download (1MB)

Abstract

The genetic relatedness among Acacia superbulk (plus tree of Acacia mangium) and Acacia hybrid (Acacia mangium x Acacia auriculiformis) collected from the UNIMAS arboretum, Kota Samarahan, Sarawak was evaluated using M13 universal Primer. A total of 16 reproducible loci was generated where 56.3% of the fragments were identified as polymorphic band with the size ranging from 500bp to 2.5kbp. An UPGMA dendrogram was constructed based on the Jaccard’s coefficient of similarity matrix which then grouped the Acacia superbulk and Acacia hybrid into two main clusters, i.e. Cluster 1: Acacia superbulk trees and Cluster 2: Acacia hybrid trees. All Acacia hybrid trees showed identical banding profile and therefore, suggested that these trees were originated from the same clone, whereas Acacia superbulk trees were originated from randomly selected plus trees seeds of A. mangium. The preliminary results showed that M13 universal primer is a potential molecular marker not only for assessing genetic relatedness but also could be used for clonal identification and seedling certification. However, further study by using other DAMD markers such as YNZ-22 and 33.6 can be carried out to validate these results.

Actions (For repository members only: login required)

View Item