Toxicity test of three commonly used herbicides on soil-dwelling ant, odontomachus simillimus and weaver ant, oecophylla smaragdina (hymenoptera formicidae)

Siti Hanisah, Binti Zahuri (2013) Toxicity test of three commonly used herbicides on soil-dwelling ant, odontomachus simillimus and weaver ant, oecophylla smaragdina (hymenoptera formicidae). [Final Year Project Report] (Unpublished)

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Abstract

A study on the toxicity test of three commonly used herbicides (glyphosate-isopropylamine, triclopyr butotyl and clethodim) against soil-dwelling ants, Odontomachus simillimus and weaver ants, Oecophylla smaragdina were tested using topical toxicity test. Different concentrations (5, 15, 25, 35, 45, 55 and 65%) with five replicates for each concentration including control were tested on both ants. Each ant was treated with one microliter (1 μL) of herbicide at the thorax. Mortality of both ants was recorded after 24 hours (2, 4, 6, 12 and 24 hours). Using probit analysis, LD50 and LD90 values of glyphosate-isopropylamine for soil-dwelling ants were 1.76% and 1.83%, triclopyr butotyl were 0.47% and 0.94%, and clethodim were 0.64% and 1.04%. LD50 and LD90 values of glyphosateisopropylamine for weaver ants were 1.56% and 1.88%, and clethodim were 1.56% and 2.27%. However, LD50 and LD90 values of triclopyr butotyl for weaver ants were unable to determine as there is 100% mortality within the 24 hours observation. In comparison, for soil-dwelling ants, triclopyr butotyl was more toxic than clethodim, and clethodim is more toxic than glyphosate-isopropylamine and for weaver ants, triclopyr butotyl was more toxic than glyphosate-isopropylamine and glyphosateisopropylamine was more toxic than clethodim.

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