Nucleotide polymorphism of enhanced disease resistance 1 (edr1) from kelampayan (neolamarckia cadamba)

Tan, Soon Yu (2013) Nucleotide polymorphism of enhanced disease resistance 1 (edr1) from kelampayan (neolamarckia cadamba). [Final Year Project Report] (Unpublished)

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Abstract

Neolamarckia cadamba (Roxb.) Bosser or Kelampayan is well-known for its contribution in economic sector whereby the tree is used as raw materials in plywood, paper and pulp industries and light construction. The enhanced disease resistance 1 (EDR1) gene controls the invasion of pathogens into the living plant, and it also serves as the negative regulator of disease resistance, ethylene-induced senescence and programmed cell death. As conventional plant breeding is costly and time-consuming, new technique such as single nucleotide polymorphism (SNP) is applied as a marker for plant breeding selection. The main objective in this study was to detect the sequence variation caused by SNP in edr1. DNA extracted from five selected Kelampayan trees were firstly subjected to polymerase chain reaction. The purified amplicons with ~550 bp were sent for DNA sequencing. However, the attempt to blast the sequenced data was failed and no significant results were found due to the weak and noise sequence data. The main reason that led to the failure was due to the contamination of purified PCR product. Gel extraction should be done in a careful manner to avoid contamination.

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