Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation

Siti Aisyah, Binti Mohd Zaman (2012) Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation. [Final Year Project Report] (Unpublished)

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Abstract

This project aims to formulate an effective microbial formulation for biodegradation of pineapple waste. A strain of fungus, Trichoderma harzianum UMAS SD17 and two strains of Bacillussp., Bacillus licheniformis UMAS P7 and Bacillus amyloliquefaciens UMAS 1002 were used with varying co-cultivation in solid state fermentation where the pineapple waste are used as the substrate. Eight analytical analysis were done to determine the most efficient microbial formulation, which includes, protein content, carbon to nitrogen ratio, biomass dry weight, glucose and reducing sugar concentration and the level of cellulose, hemicellulose and lignin degradation. Based on the data obtained, the fermentation with co-cultivation of all three microbes shows the highest cellulose degradation from 37.65% to 12.93%, and the highest hemicelluloses degradation from 6.87% to 2.36%. It also shows the second highest rate of lignin degradation after formulation of B.licheniformis and T.harzianum, from 14.5% to 9.5%. After 24 days of fermentation, this formulation also shows the closest C:N ratio to the ideal compost ratio of 25:1 at 50.12:1, thus suggesting that this formulation of microbes consisting of T.harzianum, B.licheniformis and B.amyloliquefaciens is the most efficient formulation for biodegradation of pineapple waste and can be further used for a complete composting process.

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