Biodegradation of aliphatic hydrocarbons by indigenous fungi isolated from used automobile lubricating oil

Katherine, Hii Suh Yiing (2006) Biodegradation of aliphatic hydrocarbons by indigenous fungi isolated from used automobile lubricating oil. [Final Year Project Report / IMRAD] (Unpublished)

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Abstract

Eighteen indigenous fungal isolates were successfully isolated from used motor oil, top 5 centimeters of visibly used motor oil contaminated patches of soil and drainage water contaminated with used motor oil. Among these 18 fungal isolates, SA4 (Tricho(leima asperellum strain TUB F-1067), SA5 (Trichoderma aspere//um strain Tr48), SA6 (Tricho(lernm asperellum strain TUB F-756), P1 (Penicillium species), and P9 (Aspergi/lus species) were further selected for hydrocarbon biodegradation capability analysis after preliminary screening on the average growth rate of each fungal isolate in minimal media amended with 1%(v/v) used motor oil. From these 5 fungal isolates, P1 was the isolate that has the highest potential in degrading the aliphatic hydrocarbon compounds of used motor oil. Based on the ligninolytic enzyme activity assays, P1 had secreted the highest levels of lignin peroxidase (LiP) (2.29 U/mL) and manganese peroxidase (MnP) (4.521 U/mL) that involved in the biodegradation of used motor oil. Gas chromatographic analysis further indicated that Pl was capable of degrading almost all the n-alkanes (n-C-15 to n-C�23 range) in the residual used motor oil

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