MICROPROPAGATION OF KELEMPAYAN (NEOLiMARCKIA CADAMBA (ROXB.) BOSSER)

Diana, Lim Siok Ley (2006) MICROPROPAGATION OF KELEMPAYAN (NEOLiMARCKIA CADAMBA (ROXB.) BOSSER). [Final Year Project Report] (Unpublished)

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Abstract

Kelempayan (Neolamarckia cadamba (Roxb. ) Bosser) belongs to the family Rubiaceae, is of economic importance as timber and as source of non-timber products. As a fast growing pioneer species, Kelempayan has been identified as one of the species for forest plantation and reforestation programmes in Sarawak. The objective of this study is to develop an in vitro culture method for mass propagation of this species. The study started with an attempt to establish contamination-free culture. Node, shoot tips, lamina and petiole from three-month old nursery grown seedlings were used as the explants. Results showed the explants could not withstand prolonged cleaning under running water. The explants were sensitive to surface sterilization by `Clorox®'. Most of the explants were found damaged by treatment of 15% (v/v) `Clorox®'. At 10% `Clorox®' was not effective in eliminating contaminants. PPMT"' (Plant Preservative Mixture) at 1, 2, 3, 4, 5, 10, 15 and 20 ml/l were incorporated into the medium to reduce culture contamination. The protocol below would result in 70% contamination-free shoot-tip explant in culture. The protocol involves dipping explants first in 75% ethanol for 30 seconds followed by agitating the explants in 10% `Clorox®' for 10 minutes, rinse at least three times with sterile water and culture in medium supplemented with PPM at 4 ml/1. Such protocol needs to be further refined to improve the efficiency or to be modified for other types of explants. Study on induction of multiple shoot formation was quite fruitful. Result showed that BAP at 0.5 mg/I and in combination with NAA at 0.1 mg/( and 0.3 mg/I is effective in the induction of higher shoot proliferation in nodal and shoot�tip explants respectively. It was found that a 10-week old aseptically grown seedling can provide a shoot-tip and three nodal explants for micropropagation. Using the shoot proliferation protocol developed so far a total of 13 plantlets could be produced in about six weeks after culturing the explant in the shoot induction medium

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