Chemical studies and biological activities of cinnamomum griffithii

Mohamad Termizi, Haji Piee (2008) Chemical studies and biological activities of cinnamomum griffithii. [Final Year Project Report] (Unpublished)

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Abstract

Phytochemical studies and biological activilies on cinnamomum griffithii have been conducted. The stem bark of cinnamomum griffithii was extracted using hexane, filtered and evaporated to dryness. The process was repeated using solvent with increasing polarities which yielded five crude extracts, hexane (4.48 g), dichloromethane (59.58 g), chloroform (84.68 g), ethyl acetate (23.73 g) and methanol (112.8 g). Fun her purification of dichloromethane and ethyl acetate extract using extensive column chromatography and preparative thin layer chromatography resulted in the isolation of several semi pure compounds. Toxicity against Artemia salina was performed for all the extracts and hexane extract showed significant toxicity against Artemia salina with LC50 of 100 ug/mL. Termiticidal activity against Nasutitermes sp. was also performed. At the concentration of 100 ug/mL, both dichloromethane and ethyl acetate extracts showed 100% mortality against Nasutitermes sp.. Essential oils from the leaves and stern bark of Cinnamomum griffithii and Cinnamolllum microphyllum were isolated by using hydrodistillation method and analyzed by Gas Chromatography- Flame Ionization Detector. The percentage yield of the essential oils of C. griffithii and C. microphyllum ranged between 1.19 to 3.26% and the leaves of C. microphyllum gave the highest yield. The leaves oil of C. griffithii were rich in a-copaene (15.39%), B-damascenone (13.91 %), (E.Z)-3,6-nonadien-1-01 (11.42%), and decanoic acid (10.16%) while the stem essential oil were rich in raspberry ketone (4.88%), undecanol (4.57%), citronellyl butyrate (4.5%) and methyl cinnamate (4.37%), The leaves oil of C. microphyllum were rich in p-menthatriene (13.42%), trans-sabinene hydrate (8.66%), (Z)-3-hexenyl butanoate (7.43%) and nitro-phenylethane (6.04%) while the stem oil were rich in (E)-a-bergamotene (7.63%). a-ionone (4.13%), 2-nonenal (3.61%) and sesquiphellandrene (3.32%).

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