Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay

Hung, Tze Mau (2006) Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay. [Final Year Project Report] (Unpublished)

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Abstract

The Sarawak razor clam or locally known as `Ambal' has been divided into three different species: Solen regularis Dunker 1962 (Ambal Biasa), Solen vagina Linne 1758 (Ambal Jernang) and Solen sarawakensis von Cosel. 2002 (Ambal Riong). These razor clams are well characterized by the different outlines, the arrangement of the adductor scars and pallial muscle scars. Thus, preliminary assessment on morphological characters such as weight, shell length and width were measured using the method proposed by (Pang, 1994). Moreover the arrangement of the adductor and pallial muscle scar were assessed and recorded. In comparison to other types of infaunal bivalves families such as Cardiidae and Psammobidae, the Solenacea are not species rich, but they are commercially important because there is a high demand in the seafood industries for domestic consumption. Currently, Solen spp taxonomy is solely based on morphological description. This study is an attempt to validate it using molecular data. There are problems of misidentification that therefore limit razor clams studies. Genomic DNA has been successfully extracted from the three Solen species found in Kuching Bay area using a modified version C-TAB protocol. Amplification of COI and partial cyt b mtDNA gene was unsuccessful and need further research. Modifications were done on homogenisation method and centrifugation time.

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