Sukri, Berawi (2003) Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum. [Final Year Project Report] (Unpublished)
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Abstract
(alupphYl/um lanigenum, a species that has a great potential in medicinal application, belongs to the Guttiferae family. The present study involves the isolation of DNA from this species. Three types of leaves were taken from the C. /anig>erum tree, namely matured, young and shoot leaves. The DNA isolated still contains RNA. RNase treatment was used to eliminate this impurity. The DNA obtained was stored at 20"C and 80"C up to I month and was examined tinder UV spectrophotometer to determine its concentration. Both temperatures were suitable for storage of the DNA without degradation. Storage of the DNA at such temperature could facilitate the isolation of useful genes from the plant for biotechnological applications.
| Item Type: | Final Year Project Report |
|---|---|
| Additional Information: | Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2003. |
| Uncontrolled Keywords: | Calophvllum lanigerum, DNA isolation, DNA concentration |
| Subjects: | S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Unai |
| Date Deposited: | 13 Nov 2024 06:51 |
| Last Modified: | 13 Nov 2024 06:51 |
| URI: | http://ir.unimas.my/id/eprint/46617 |
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