Mohd Aminudin, Mustapha and Sim, Sai Peng (2024) Green Fluorescent Proteins (GFP) Induce Apoptosis in Normal Nasopharyngeal Cells Expression. International Medical Journal, 31 (4). pp. 117-119. ISSN 1341-2051
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Abstract
Objective: To evaluate the effect of Green fluorescent proteins (GFP) expression on apoptosis in normal nasopharyngeal cells. Material and Methods: pTracer vector that contained GFP expression and pcDNA transfected into normal nasopharyngeal cell line. Apoptosis was accessed by measuring Caspase activities using Caspase-Glo ® 3/7 Assay kit following the manufacturer's protocol. The luminescence intensity was measured by microplate reader. Association of GFP expression with caspase activation was analysed by independent Sample T test. Results: The association between caspase activity and GFP expression are statistically significant compared to pcDNA vector control cells. Conclusion: Our results show that there is an association of GFP expression in pTracer vector with caspase activity in normal nasopharyngeal cell thus support that GFP expression induce apoptosis in normal nasopharyngeal cell.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GFP, pTracer, pcDNA, Caspase Activity Nasopharyngeal Cell. |
| Subjects: | Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General) |
| Divisions: | Academic Faculties, Institutes and Centres > Centre for Pre-University Studies Faculties, Institutes, Centres > Centre for Pre-University Studies Academic Faculties, Institutes and Centres > Centre for Pre-University Studies |
| Depositing User: | Mustapha |
| Date Deposited: | 09 Oct 2024 03:23 |
| Last Modified: | 09 Oct 2024 03:23 |
| URI: | http://ir.unimas.my/id/eprint/46259 |
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