Synthesis and molecular docking simulation on the antimicrobial efects of halogenated vanillin‑azo dyes and schif base derivatives

Mohamad Azmeer, Hissam and Zainab, Ngaini and Nor Hisam, Zamakshshari and Fatin Nur Amira, Mohammad Hejemi and Fatin Salihah, Arni and Ainaa Nadiah, Abd Halim (2024) Synthesis and molecular docking simulation on the antimicrobial efects of halogenated vanillin‑azo dyes and schif base derivatives. Discover Applied Sciences, 6 (325). ISSN 3004-9261

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Abstract

Lead compounds containing nitrogen pharmacophores from natural resources have garnered interest among researchers due to their potential for drug development. However, the extractions of the active metabolites are usually labor-intensive and time-consuming. In this study, halogenated vanillin derivatives featuring azo dyes (N=N) (1a-1 h) and Schif base (C=N) (2a-2 h) have been synthesized via diazonium coupling and nucleophilic substitution reaction, respectively. The comparative efect of N=N and C=N moieties was evaluated for antibacterial properties against Staphylococcus aureus and Escherichia coli via disc difusion method. Incorporating C=N (8–13 mm) into the vanillin network showed excellent inhibition against S. aureus compared to N=N (7–8 mm) and the standard ampicillin (12 mm). While the halogenated vanillin featuring N=N (7–9 mm) and C=N (7–8 mm) moieties showed excellent zone of inhibitions against E. coli compared to the parent vanillin. The in-silico screening using AutoDock Vina, showed 2c-h (inhibition zone>10 mm) with a high binding affinity against DNA gyrase enzyme with binding energy ranging from − 7.3 to − 7.9 kcal/mol, similar to re-docking of ampicillin − 7.6 kcal/mol and co-crystalize compounds BPH651 with − 7.5 kcal/mol. This research contributes a signifcant milestone in drug design, especially for the development of new antibacterial drugs with outstanding properties.

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