CD4-1 and CD4-2 single positive are two major CD4 lymphocyte subpopulations in ginbuna crucian carp Carassius auratus langsdorfii

Lik Ming, Lau and Misato, Kuga and Motohiko, Sano and Goshi, Kato (2023) CD4-1 and CD4-2 single positive are two major CD4 lymphocyte subpopulations in ginbuna crucian carp Carassius auratus langsdorfii. Developmental & Comparative Immunology, 148. p. 104968. ISSN 1879-0089

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Abstract

Fish possess two divergent forms of CD4: CD4-1 resembles the mammalian CD4 whereas CD4-2 is a unique CD4 homolog. Three CD4+ cell subpopulations were identified in fish: CD4-1 single-positive (SP), CD4-2 SP, CD4-1/CD4-2 (CD4) double-positive (DP). With the aid of monoclonal antibodies (mAbs) against CD4-1 (6D1: provided by Prof. Dr. Teruyuki Nakanishi) and CD4-2 (D5) of ginbuna, tissue distribution of CD4-1 SP, CD4-2 SP, and CD4 DP lymphocyte subsets were investigated in ginbuna. Leukocytes from peripheral blood, trunk-kidney, head-kidney, spleen, and thymus of ginbuna were stained with the mAbs 6D1 (FITC-conjugated anti-rat IgG2a) and D5 (APC-conjugated anti-mouse IgM). Rat IgG2a and mouse IgM isotype were used as the negative controls against the mAbs. The antibody-stained cells were further nuclear stained with DAPI to exclude the non-viable cells. Then, a lymphocyte gate was made in the viable cells and then further analyzed by flow cytometry. Two-color immunofluorescence analysis with 6D1 and D5 by flow cytometry revealed that the percentages of CD4-1 SP and CD4-2 SP lymphocytes were comparatively higher than CD4 DP lymphocytes in all tissues examined in ginbuna. The highest percentage of CD4-2 SP lymphocytes (~40%) was found in the thymus, while the head-kidney exhibited the highest percentages of CD4-1 SP (~30%) and CD4 DP (~5%) lymphocytes. The highest and lowest proportions of total CD4+ lymphocytes were observed in the thymus (~67%) and PBL (~26%), respectively. These findings indicated that ginbuna CD4+ lymphocyte population consists of two major subpopulations (CD4-1 SP and CD4-2 SP) and a minor subset (CD4 DP).

Item Type:	Article
Uncontrolled Keywords:	CD4 lymphocyte, Carassius auratus langsdorfii, CD4-1 and CD4-2.
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:	Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User:	Lik Ming
Date Deposited:	11 Jul 2024 00:42
Last Modified:	11 Jul 2024 00:42
URI:	http://ir.unimas.my/id/eprint/45206

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• CD4-1 and CD4-2 single positive are two major CD4 lymphocyte subpopulations in ginbuna crucian carp Carassius auratus langsdorfii. (deposited 11 Jul 2024 00:42) [Currently Displayed]

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