Analysis of metoprolol enantiomers via reverse phase (RP-HPLC) with M - Cyclodextrin as mobile additive

Asiah, Zulkifli and Mariani, Rajin and Sariah, Abang and S. M., Anissuzzaman and Azlina, Harun@ Kamaruddin (2020) Analysis of metoprolol enantiomers via reverse phase (RP-HPLC) with M - Cyclodextrin as mobile additive. Journal of Physics: Conference Series, 1529 (4). pp. 1-8. ISSN 1742-6596

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Abstract

Enantiomeric separation of the racemic metoprolol was investigated using a reverse phase HPLC (RP-HPLC) with Methyl-beta-cyclodextrin (M-B-CD) as chiral mobile phase additive. A comparison of the enantioseparation of the racemic metoprolol, the system suitability, linearity, accuracy, limit of detection and quantification was undertaken to show the performance between two C18 columns, a Zorbax Eclipse XDB C-18 column (15 cm x 10 mm, 10 μm) and a Syncronis C18 HPLC column (250 mm x 4.6 mm, 5.0 μm) using high performance liquid chromatography with the same condition of mobile phase composition, pH value of the mobile phase and concentration of chiral additives. The resolution was achieved using a mobile phase consisting of a mixture of aqueous solution (3.5 g M-�-CD in 300 ml H20), methanol with a volumetric ratio of 86:14 (v/v) and a flow rate of 1.0 ml/min and 0.5 ml/min for the columns respectively. Conversion of S-metoprolol found in this study are 60, 51, 15. © Published under licence by IOP Publishing Ltd.

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