Mohamad, Khairulmunir and Millawati, Gani and Kayal Vizi, Karuppannan and Abd Rahman, Mohd Ridwan and Badrul Munir, Md Zain (2023) High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting. Biodiversity Data Journal, 11 (e1047). pp. 1-22. ISSN 1314-2828
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Abstract
The Malayan tiger (Panthera tigris jacksoni) is a critically endangered species native to the Malaysian Peninsula. To imitate wild conditions where tigers do not hunt every day, numerous wildlife sanctuaries do not feed their tigers daily. However, the effects of fasting on the gut microbiota of captive Malayan tigers remains unknown. This study aimed to characterise the gut microbiota of captive Malayan tigers by comparing their microbial communities during fasting versus normal feeding conditions. This study was conducted at the Melaka Zoo, Malaysian Peninsula and involved Malayan tigers fasted every Monday. In total, ten faecal samples of Malayan tiger, two of Bengal tiger (outgroup) and four of lion (outgroup) were collected and analysed for metabarcoding targeting the 16S rRNA V3–V4 region. In total, we determined 14 phyla, 87 families, 167 genera and 53 species of gut microbiome across Malayan tiger samples. The potentially harmful bacterial genera found in this study included Fusobacterium, Bacteroides, Clostridium sensu stricto 1,Solobacterium, Echerichia shigella, Ignatzschineria and Negativibacillus. The microbiome in the fasting phase had a higher composition and was more diverse than in the feeding phase. The present findings indicate a balanced ratio in the dominant phyla, reflecting a resetting of the imbalanced gut microbiota due to fasting. These findings can help authorities in how to best maintain and improve the husbandry and health of Malayan tigers in captivity and be used for monitoring in ex-situ veterinary care unit.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Panthera tigris jacksoni, carnivora, next generation sequencing, 16S rRNA, metabarcode. |
| Subjects: | Q Science > QL Zoology |
| Divisions: | Academic Faculties, Institutes and Centres > Centre for Pre-University Studies Faculties, Institutes, Centres > Centre for Pre-University Studies Academic Faculties, Institutes and Centres > Centre for Pre-University Studies |
| Depositing User: | Abd Rahman @ Tahir |
| Date Deposited: | 06 Sep 2023 00:13 |
| Last Modified: | 03 Oct 2023 00:20 |
| URI: | http://ir.unimas.my/id/eprint/42738 |
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