Production and Characterisation of Laccase from Fungi by using Leaf Litter as Substrate

Gary, Hogres (2022) Production and Characterisation of Laccase from Fungi by using Leaf Litter as Substrate. [Final Year Project Report] (Unpublished)

[img] PDF (Please get the password by email to repository@unimas.my , or call ext: 082-583914/3973/3933)
GARY HOGRES.pdf
Restricted to Registered users only
Download (3MB)

Abstract

The interest of laccase enzyme as a promising biocatalyst in various applications has been grow extensively throughout the years. Various fungi especially from phylum Basidiomycota and white-rot fungi has shown to produce laccase enzyme. Serial dilution was carried out to isolate laccase producing- fungi from leaf litter and undergoes screening for laccase oxidation by using guaiacol and 0.01% chloramphenicol. SSF method was carried out and from utilising leaf litter as substrate to provide more economical and environmentally-friendly. The laccase activity were measured and fungal isolate, Pyrrhoderma lamaense has the highest laccase activity 0.06042 (U/g) with optimum pH of 9. Visualisation of isoforms were taken from native-SDS PAGE for the fungal samples. BLAST analysis was carried out to search match DNA sequence in GenBank database. The data sample scores 100% for Pyrrhoderma lamaense, from phylum Basidiomycota with its purified molecular weight of 750bp.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2022.
Uncontrolled Keywords: Laccase, Basidiomycota, white-rot fungi, leaf litter, guaiacol, Pyrrhoderma lamaense, native- SDS PAGE.
Subjects: Q Science > QP Physiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Dan
Date Deposited: 22 Sep 2022 03:49
Last Modified: 14 Sep 2023 07:01
URI: http://ir.unimas.my/id/eprint/39938

Actions (For repository members only: login required)

View Item View Item
UNIMAS Institutional Repository is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.