Characterization of Bacteria Isolated from Waters from Rivers of Kampung Paku, Betong

Valintiny, Patrick (2022) Characterization of Bacteria Isolated from Waters from Rivers of Kampung Paku, Betong. [Final Year Project Report] (Unpublished)

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Abstract

Bacteria isolated from the water environment are mostly Gram-negative bacteria. These Bacteria can either be pathogenic or non-pathogenic bacteria. Considering that the water environment plays a major role for communities living in residential areas. It is important to ensure that the water quality in this area is not contaminated. This study aims to determine and characterize the bacteria in water samples from rivers within Paku village area in Betong, Sarawak. There were 15 samples cultured on Nutrient agar which were then identified by using 16S rDNA Sequencing. The bacteria isolates were characterized by using AGE and tested against eight types of antimicrobial agents. Based on the result, there were five family of bacteria identified, which were Enterobacteriaceae, Aeromonadaceae, Moraxellaceae, Bacillaceae, and Nesseriaceae. Some of the bacteria identified were coliform bacteria, for instance Escherichia coli, which indicates that there might be contamination in the water samples. From the MAR index, only 38.46% of bacteria isolates have a value lower than 0.2, whereas 30.77% has a value higher than 0.2. This study reveals the possibility of contamination in the water sample.

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