Optimisation of SSF Parameters for Cellulase Production by Aspergillus spp. Via Solid-State Fermentation Using Agro-Waste as the Substrate

Thong, Kah Huey (2022) Optimisation of SSF Parameters for Cellulase Production by Aspergillus spp. Via Solid-State Fermentation Using Agro-Waste as the Substrate. [Final Year Project Report] (Unpublished)

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Abstract

Malaysia produced few thousand metric tons of agro waste annually; the improper waste disposal causes environmental pollution. Thus, bioprocess industry should take agro waste as the substrate for bioprocess to produce enzyme such as cellulase. Cellulase is known as an economical important enzyme in various industries. As the demand for cellulase is increasing due to the development of biofuel industry, the optimisation of solid state fermentation parameters to produce high quality cellulase with lower production cost and higher efficiency need to be studied. In this study, Aspergillus niger was used to perform SSF with corn husk as substrate to produce cellulase. The cellulase activity at different optimised SSF parameters such as initial moisture content, incubation period and incubation temperature were assayed by filter paper activity (FPA) assay. The FPA was used to test the cellulase activity and the amount of glucose released. The optimum temperature for the highest cellulase enzyme activity of A. niger grown on corn husk was at 30°C while optimum initial moisture content was 65% (v/w) and 6 days was the optimum incubation period. The highest enzyme activity was 47.2222 ± 5.6 U/ml. The results suggest the potential of corn husk to be utilised for industrial purpose to produce cellulase using A. niger.

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