Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)

Soonali, Wasdewa (2022) Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs). [Final Year Project Report] (Unpublished)

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Abstract

Foods contain fats, oils, and greases (FOGs) are commonly washed down into the kitchen sink and sticks to the inner walls of kitchen pipes, drains and sewer pipes and forming clogs which causes the blockage that restrict the flow of wastewater. Waste FOGs material sometimes flows directly to the mainstream and forms an oil layer which give threat to aquatic organisms by reducing the dissolved oxygen level. The biosurfactant producing bacteria act as biodegradation agent to emulsify the FOG materials from clogging and prevent blockage in the drains and sewage system. This study therefore aims to isolate and identify the biosurfactant producing bacteria. The oil spreading assay and emulsification index (E24) test were used to determine the presence of biosurfactant and Bacillus paramycoides and Bacillus cereus were identified via comparison of the isolates’16S rRNA with deposited sequences in NCBI GenBank. Bacillus paramycoides and Bacillus cereus produced largest diameter of clearing zone compared to other biosurfactant producing bacteria on the oil spreading assay which indicated as the high quality of biosurfactants produced. Biosurfactant screening via emulsification index (E24) increased with presence of biosurfactants produced by B. paramycoides and Bacillus cereus due to the high interaction between water and oil which have industrial potential with greater ability in biodegrading the FOGs materials.

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