Effect of Ammonium Sulphate on Bioethanol Production from Banana Hydrolysate

Sandi, Lau Chien Chien (2022) Effect of Ammonium Sulphate on Bioethanol Production from Banana Hydrolysate. [Final Year Project Report] (Unpublished)

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Abstract

Banana is an important and one of the earliest crops produced in the agriculture history. However, its harvest causes a huge number of agricultural wastes. In order to avoid environmental issues, banana agro wastes was utilised to produce value-added products such as bioethanol. The information on the influence of nitrogen sources on bioethanol production from banana hydrolysate is still limited. Therefore, this study was carried out to study the effect of ammonium sulphate (NH4)2SO4, as a nitrogen source on the bioethanol production from banana hydrolysate. The banana stems were hydrolysed using liquozyme and spirizyme enzymes producing banana hydrolysate. The resulting banana hydrolysate was used as a substrate for bioethanol production by Saccharomyces cerevisiae. Different concentrations of ammonium sulphate were applied, namely 0, 2, 4, and 6 g/L. The fermentation performance was analysed in terms of cell growth, substrate consumption, and bioethanol production. The results showed that the addition of 2 g/L of ammonium sulphate to the fermentation resulted in the highest bioethanol concentration, 6.65 g/L, which was 4-fold over that in the control experiment. Additionally, the glucose consumption was the highest, 98.12%. The results indicated that the introduction of ammonium sulphate in the banana hydrolysate fermentation caused a positive effect on the bioethanol production.

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