Expression Analysis of Differentiated Sago Palm Gene (Sulfate Transporter and Boron Transporter) via Amplified cDNA

Samuel, Ng Chun Keat (2022) Expression Analysis of Differentiated Sago Palm Gene (Sulfate Transporter and Boron Transporter) via Amplified cDNA. [Final Year Project Report] (Unpublished)

	PDF SAMUEL NG CHUN KEAT 24pgs.pdf Download (559kB)
	PDF (Please get the password by email to repository@unimas.my, or call ext: 3914/ 3942/ 3933) Samuel Ng ft.pdf Restricted to Registered users only Download (1MB)

Abstract

The scientific name of the sago palm is Metroxylon sagu. It is a species that can be found in wetland areas of Malaysia. The present study looks on the gene expression level of sulfate transporter and boron transporter gene by using trunking and non-trunking sago palm leaves. Liquid nitrogen was used to deactivate the nucleases in the leaves. CTAB extraction method was used for RNA extraction. DNase treatment was carried out to increase the yield of RNA. Primers were designed for targeting sulfate transporter gene and boron transporter gene. Gel electrophoresis was done for visualize the RNA, fragments then convert to cDNA and undergo amplification. The expression level of sulfate transporter gene and boron transporter gene was found to be similar in trunking and non-trunking sago trees. Thebands in sulfate transporter gene were more fainted when compared to boron transporter gene. Further research on the gene expression level of both gene should be conducted in order to understand the formation of non-trunking sago palm trees and due to its high potential in economic application.

Actions (For repository members only: login required)

View Item