Utilisation of Leaf Litter from Cinnamomum iners (Wild Cinnamon) for Laccase Production by Marasmius clodophyllus UMAS MS8

Nur Hannah Samirah, Saini (2022) Utilisation of Leaf Litter from Cinnamomum iners (Wild Cinnamon) for Laccase Production by Marasmius clodophyllus UMAS MS8. [Final Year Project Report] (Unpublished)

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Abstract

Laccase is still not widely applied in industry because of the limitation in its production. Production of laccase is very expensive by using chemical media. As a consequence of that, researchers opt for alternative ways to produce laccase affordably by using agricultural waste such as wheat bran, oil cakes and fruit peels which are easy to find and affordable. In this research, Marasmius cladophyllus UMAS MS8 was examined for its ability to produce laccase by using Cinnamomum iners as a substrate by using two fermentation methods, which were submerged fermentation (SmF) and solid-state fermentation (SSF). The fungi were maintained on 2% (w/v) malt extract broth solution at 4 ℃. Fermentation was carried out with 10 g of C. iners and glucose minimal media as the liquid medium in two fermentation methods. In SSF , glucose minimal (GM) media was used to moisten the C. iners to 70% (v/w). However, in SmF, 100 mL of the media was added to the C. iners. The fermentation process was carried out for 12 days with sampling at three-day interval. Laccase activity was determined based on 1 mmol of 2,2- azino-bis-[3-ethylthiazoline-6-sulfonate] (ABTS) assay. The result obtained showed that M. cladophyllus could not produce laccase in both SSF and SmF. However, if to compare between these two methods, SSF was more favourable condition for the M. cladophyllus to grow since the laccase reading of SSF (0.0285 U/g) was higher than SmF (0.00574 U/g) method after 12 days of fermentation. Bradford assay was obtained to confirm that there were protein produced by the M. cladophyllus but not laccase.

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