Expression Analysis of Differentiated Sago Palm Gene (Nuclear transcription factor Y subunit C and Cytochrome P450) via Amplified cDNA

Muhammad Ilyas, Arsad (2022) Expression Analysis of Differentiated Sago Palm Gene (Nuclear transcription factor Y subunit C and Cytochrome P450) via Amplified cDNA. [Final Year Project Report] (Unpublished)

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Abstract

Metroxylon sagu Rottbol is an important type of palm that contributes to economic value in Malaysia. However, sago palm grown on peat soil were found to be non-trunking and produce lower quality of sago. The present study looks at the gene expression analysis of NF-YC and cytochrome P450 genes specifically towards the band intensity. Total RNA of trunking and non-trunking sago palm was extracted using CTAB extraction buffer. Using RT-PCR, the RNA is converted to cDNA an amplified using PCR. An amplicon of 347 bp size was produced for the NF-YC gene. Relative intensity values for non-trunking sago palm were calculated using ImageJ, which was found to be higher than trunking sago palm. Differentially expressed genes can be examined to understand the possible function leading to specific physiological traits. Further studies on the gene expression of the NF-YC gene should be conducted to explain the physiological characteristics of non-trunking sago palm.

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