De novo shoot regeneration of Neolamarckia cadamba (Roxb.) Bosser

Mohd. Azrin, Abdullah (2022) De novo shoot regeneration of Neolamarckia cadamba (Roxb.) Bosser. [Final Year Project Report] (Unpublished)

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Abstract

Neolamarckia cadamba (Roxb.) Bosser, locally known as Kelampayan, is a multipurpose tree species that has the ability to grow faster than many other tropical timber species, which can help in reducing the impact of deforestation, as well as to continuously cater the raw materials for commercial purposes. Despite many studies have been conducted on de novo shoot regeneration, the application of this method on N. cadamba is limited, and thorough studies on the process is needed to diversify in vitro regeneration methods for N. cadamba. Also, lack of research has been done to study the effects of different thidiazuron (TDZ) concentrations on de novo shoot regeneration for N. cadamba. This study focused on generating N. cadamba through de novo shoot regeneration and determining the optimum TDZ concentration for N. cadamba using leaf explants. Three different TDZ concentrations (2.0, 4.0 and 6.0 mgL-1) were tested, focusing on three factors, which are the length of leaf explants, leaf counts and shoot counts. After 5 weeks of culturing, 6.0 mgL-1 TDZ exhibited the best results, posting the highest mean length of leaf explants, leaf counts and shoot counts. However, it is not enough to conclude that the concentration stated is the optimum TDZ concentration for N. cadamba. Based on the preliminary results, the method can be further improved to fully utilise TDZ as it becomes more common to be used to induce a systematic micropropagation for woody plant species. Thus, by determining the optimum TDZ concentration for N. cadamba, it can be used as an indicator to determine the optimum TDZ concentration for other tropical timber species.

Item Type:	Final Year Project Report
Additional Information:	Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2022.
Uncontrolled Keywords:	Neolamarckia cadamba, de novo, shoot regeneration, thidiazuron, leaf explants
Subjects:	Q Science > QK Botany
Divisions:	Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User:	Dan
Date Deposited:	20 Sep 2022 04:35
Last Modified:	11 Jan 2024 07:31
URI:	http://ir.unimas.my/id/eprint/39851

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