Comparative Leaf Proteomes of Irradiated Mutant and Wild Type Oryza sativa subsp. indica

Mohd Khairul Akma, Darwis (2022) Comparative Leaf Proteomes of Irradiated Mutant and Wild Type Oryza sativa subsp. indica. [Final Year Project Report] (Unpublished)

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Abstract

Radiation is a common phenomenon that occurs in the natural setting which leads to mutation occurrence in various organisms. In the plant kingdom, particularly, the occurrence of mutation is unnecessarily reflected as a negative thing. However, for over centuries, humanity has inverted perspectives on mutation by labelling mutation as a defect in any organism and conjecturing mutation solely gave disadvantages for humankind. Therefore, this study adapted the proteomics approach in investigating the comparison between irradiated mutant and wild type of Oryza sativa subsp. indica at proteome level. The leaf samples were prepared by using a conventional method of grinding in liquid nitrogen by using mortar. The adoption of PEG fractionation technique in this study ensured an efficacious way of protein retrieval from samples. The use of Bradford protein assay provided a close approximation of protein concentration in the sample. The adoption of SDS-PAGE helped in separation of proteins according to their molecular weight. The gel result from SDS-PAGE revealed that separation of 13 proteins from the supernatant sample ranged from 10 to 74 kDa for both rice types whereas for the pellet sample separated 8 proteins ranged from 10 to 45 kDa. In addition, pellet samples for both rice types are unable to resolve a clear band separation due to low protein abundance with 5 protein bands unable to be detected as compared to supernatant samples. The obvious difference only the band thickness at Band 5 (42 kDa) between wild type and mutant type which suggested there is alteration of protein production occurred in mutant type.

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