Gene Sequence Analysis of Tumor Susceptibility Gene 101 (TSG101) in Nasopharyngeal Cancer Cell Lines

Michelle, Yew Zhi Yan (2022) Gene Sequence Analysis of Tumor Susceptibility Gene 101 (TSG101) in Nasopharyngeal Cancer Cell Lines. [Final Year Project Report] (Unpublished)

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Abstract

TSG101 acts as a tumor suppressor gene. Most research also indicates that TSG101 may have a variety of functions in carcinogenesis as well as the development of tumors in diverse cell types. The recent findings proved that mutations of the TSG101 gene were detected in many cancer diseases such as ovarian carcinomas, human papillary thyroid carcinomas, colorectal cancer, and others. Furthermore, a deletion known as TSG101 Del 154-1054 was discovered in the NPC cell line (TW01). Despite this, research on nasopharyngeal carcinomas (NPC) cell line mutations such as insertions, duplication, and others were still restricted. The objectives of this study are to analyse and compare the TSG101 gene sequence between the NCBI reference sequence and the NPC cell lines TW01 and EBV infected C666-1, as well as to observe the other types of mutation or genetic lesions in the TSG101 gene in the context of NPC. The starting materials were total RNA from TW01 and C666-1, which were then transcribed into cDNA using M-MLV RT. The cDNA was then amplified using polymerase chain reaction and then proceed with sequence analysis. There were bands identified between 200 and 300bp, indicating that TSG101 amplicon. The PCR product for these studies was supposed as a part of UEV. However, the result of this study are inconclusive, since the objectives of these studies cannot proceed due to lack of sequencing because the amplified TSG101 was not sent for sequencing in time.

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