Microbial Profiling of Dropsy Diseased Empurau (Tor tambroides)

Fong, Jia Khi (2022) Microbial Profiling of Dropsy Diseased Empurau (Tor tambroides). [Final Year Project Report] (Unpublished)

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Abstract

The intentions of this study were to identify and compare the microbial community between skin samples of dropsy diseased and health Empurau to reveal the potential pathogen of dropsy disease which reduce yield by increasing the mortality of Empurau. Skin samples of healthy and dropsy-diseased fish was harvested and subjected to serial dilution before they were plate on different agar (Aeromonas agar, nutrient agar, EMB agar, MacConkey agar). The colonies formed on the plates were picked as much as possible based on their morphological difference and inoculated onto the patch plate for single colony isolation. A total of 10 isolates from healthy fish skin samples (Aeromonas agar: 4; nutrient agar: 2; EMB agar: 3; MacConkey agar: 3) and 12 isolates from diseased fish skin sample (Aeromonas agar: 3; nutrient agar: 2; EMB agar: 2; MacConkey agar:3) were then identified by morphological characteristics, Gram staining and string test. Among these 22 isolates, only one was Gram positive coccus and the 21 isolates were all Gram negative rod-shaped bacterial. The isolates also subjected to PCR and agarose gel electrophoresis before sending them for molecular 16s rDNA sequencing which targeting the V3-V4 region with 450 bp to 550 bp. Overall, there were 10 types of different bacteria species had been identified. They were from genus of pseudomonas (59.0%), Citrobacter (32.0%) and Staphylococcus (4.5%) with one uncultured bacterium (4.5%) which might be resulted from mixed cultured of colony. Further research on the above-mentioned potential pathogen for dropsy disease should be conducted to maintain the high yields of empurau to maximise profit.

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