Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines

Bonnie, Tang Bao Chee (2022) Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines. [Final Year Project Report] (Unpublished)

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Abstract

Colorectal carcinoma, or colorectal cancer (CRC), is a type of cancer that affects the colon or rectal area and it is likely to be related with TSG101 gene expression. TSG101 is a gene that encodes a multi-domain protein that is homologous to an inactive ubiquitin-conjugating enzyme and it may act as a positive enhancer in carcinogenesis and CRC progression. In the recent finding on the TSG101 has shown to be high expression in CRC, but there are no studies that have been done in determining and analyzing the gene sequences of TSG101 in CRC cell lines. The objectives of this study are to determine the existence of TSG101 in CRC cell lines and to perform In Silico Characterization of TSG101 in CRC cell lines. The starting materials of this study were the total RNA that was isolated from HCT116 and HT29 cell lines, and they were reverse transcribed into cDNA to perform PCR. Lastly, the amplified products proceeded for sequencing and analysis. However, due to some unavoidable errors, we could not send our PCR product on time. The PCR products showed expression in the expected size range (200-300bp), hence the products were likely to be TSG101 in HCT116 and HT29 cell lines.Therefore, we subjected the expected amplified sequence (NM_006292.4) on CD search and we found out that the predicted amplified product is located at the nucleotide 499 to 739 in TSG101 and it is a region of UEV domain.

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