Isolation and Characterisation of Lipogenesis Inducing Factor in Enkabang (Shorea macrophylla)

Aida, Chan Hui Hui (2022) Isolation and Characterisation of Lipogenesis Inducing Factor in Enkabang (Shorea macrophylla). [Final Year Project Report] (Unpublished)

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Abstract

Shorea macrophylla or Enkabang is an important feedstock used by Sarawak farmers as finishing feed to induce deposition of intramuscular fats (IMF) in livestock animals. The present study focuses on extracting the potential agonists or potential PPARγ ligands from Enkabang and characterise its efficiency in inducing lipogenesis in livestock animals. A total of 218 compounds were successfully identified with GC-MS when extracted with acetone, isopropanol and diethyl ether solvents. Out of 218 potential ligands, approximately 98 of them shown hydrogen bonding to PPARγ model structure and only a selected few have been considered as a possible agonist of PPARγ. Among the 98, the compound that achieved the highest affinity score obtained from molecular docking analysis was -9.1 kcal/mol. Three compounds identified (2H-Benzo[d][1,3]oxazine, 2-[2-(5-methylfuran-2-yl)ethyl]-4,4-diphenyl-1,4-dihydro- compound, ergostane-3,6-dione, 25-(acetyloxy)-5-hydroxy-, (5.alpha.)- compound, and acetamide, N,N'-(methylenebis(2-chloro-4,1-phenylene))bis- compound) extracted with solvent diethyl ether were speculated to have a potential to be PPARγ agonists since it hydrogen bonded to amino acid residues GLN286, ARG288, SER289 and SER342, similar to positive control ROSI. Further research on other lipogenesis-related protein receptors and verification of the three potential agonists in inducing lipogenesis should be conducted due to its great potential as finishing feed in inducing IMF deposition in livestock animals.

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