Muhammad Afif, Ruzaidi (2022) Comparative In Silico Analysis of Serine/Threonine Protein Kinase in Enterococcus faecalis. [Final Year Project Report] (Unpublished)
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Abstract
Enterococcus faecalis is a nosocomial pathogen that possess resistant against multiple antibiotics. Serine/Threonine Protein Kinase, Stk has been linked to a variety of traits such as biofilm formation, cell wall biosynthesis, stress responses, metabolic pathways, autolysis, and virulence. Some studies have demonstrated comparative genome analysis in E. faecalis from different origin, however none have demonstrated comparative in silico analysis of the stk gene in E. faecalis from different origin which could accommodate details regarding the pathogenicity of E. faecalis strains. The goal of this research is to evaluate the phylogeny of E. faecalis strains from different origin using 16S rRNA and stk, and to determine the domain of Stk E. faecalis strains from different origin, as well as to evaluate virulence profiles of E. faecalis strains from different origin. This study analyses the phylogenetic tree, domain of Stk, and virulence genes of E. faecalis strains from different origin. The phylogenetic tree of stk E. faecalis was obtained and showed that it was better to differentiate E. faecalis origin compared to the phylogenetic tree of 16S rRNA using Molecular Evolutionary Genetics Analysis (MEGA) software. The domains of Stk E. faecalis strains were obtained using Simple Modular Architecture Research Tool (SMART) and it was found that PASTA (2) domain has different Expected Value (E-value) for E. faecalis strains from different origin. The virulence genes were obtained using Virulence Factor Database (VFDB), VirulenceFinder, ResFinder, and Comprehensive Antibiotic Resistance Database (CARD) and compared with E. faecalis strains from different origin. Virulence genes originated from human and animal were different from the presence of virulence in other isolates. From this research, the information regarding the stk gene could be used as a target gene among healthcare providers that are effective to control enterococcal infections in the future.
| Item Type: | Final Year Project Report |
|---|---|
| Additional Information: | Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2022. |
| Uncontrolled Keywords: | Serine/Threonine Protein Kinase, Enterococcus faecalis, virulence gene, bioinformatics |
| Subjects: | Q Science > QP Physiology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Dan |
| Date Deposited: | 12 Sep 2022 02:35 |
| Last Modified: | 10 Jan 2024 02:28 |
| URI: | http://ir.unimas.my/id/eprint/39624 |
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