Screening and Evaluation of Lipid Degrading Microbes for Potential Bioremediation of Lipid-Contaminated Waste

Foo, Huey Ci (2022) Screening and Evaluation of Lipid Degrading Microbes for Potential Bioremediation of Lipid-Contaminated Waste. [Final Year Project Report] (Unpublished)

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Abstract

A common contaminant that can be found easily in waste is lipids. Hence, lipid degrading microbes play an important role in lipid degradation. This project is conducted to screen lipid degrading microbes and determine the best lipid degrading microbe in order to evaluate its potential in the biodegradation of actual lipid-contaminated samples. A total of three microbes, Pseudomonas aeruginosa, Serratia marcescens, and Bacillus amyloliquefaciens are selected for this project. Identification of the microbes was done by gram stain test and observation under a light microscope. The three microbes showed positive results in both screening of lipid degrading microbe tests by using phenol red agar and Tween 80 agar. Microbial consortia of P. aeruginosa and B. amyloliquefaciens showed the highest lipase activity (44%) in the emulsification index test. Bioremediation of the microbial consortium was done for 28 days in two contaminated wastewater samples from different locations. The evaluation of the microbial consortium in the biodegradation of lipid-contaminated samples was evaluated by using the gas chromatography-mass spectrometry (GC-MS) technique. Five chemical compounds, dotriacontane (31.17%), heneicosane (2.81%), octadecanoic acid (0.22%), tetracontane (5.62%), and triacontane, 1-iodo- (10.39%) were successfully degraded by microbial consortia of P. aeruginosa and B. amyloliquefaciens in wastewater sample from the kitchen.

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