Effects of salt stress on total phenolic content tomato genotype Lycopersicon esculentum var MT1

Aimie Shalmee, Apendi (2022) Effects of salt stress on total phenolic content tomato genotype Lycopersicon esculentum var MT1. [Final Year Project Report] (Unpublished)

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Abstract

Salinity level is a significant limitation in abiotic factors that limits the growth, physiology and development of plants which result in major loss to the agriculture yield. Plants experience substantial secondary stressors due to salinity, including nutritional shortage, osmotic, and oxidative damage resulting in a hazardous accumulation of sodium and chloride thus affecting water absorption. Unsustainable agriculture also contributes to an increase in salinized land. In this research, seedlings pot experiment was carried out with a tomato cultivar to investigate the effect of sodium chloride (NaCl) on plant development and phenolic content under different concentration of salt stress. For the salinity study, 0, 125 and 150 mM of NaCl were applied to the tomato seedlings. All the seedlings were harvested after 14 days germination. The green parts which were the leaves and shoots including the roots of the tomato plants were weighed for phenolic content analysis. The phenolic content (TPC) was analysed by using Folin-Ciocalteu method with gallic acid as standard to form the calibration curve. In this study, excess salt treatment also been highlighted. The results showed that salt treatment with 150 mM concentration has the highest TPC (8.13 ± 4.7 mg GAE/g DW) while plants treated with 0 mM and 125 mM has TPC of 1.31 ± 0.75 mg GAE/g DW and 6.50 ± 3.8 mg GAE/g DW respectively.

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