Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates

Son, Radu and Samuel, Lihan and Azhar, Idris and Oii, Wai Ling and Muthafar, Husein Al-Haddawi and Gulam, Rusul (1999) Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates. Southeast Asian Journal of Tropical Medicine and Public Health, 30 (4). pp. 760-763. ISSN 2697-5718

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Abstract

Seven isolates of Burkholderia pseudomallei from cases of melioidosis in human (2 isolates) and animal (2 isolates),cat (one isolate) and from soil samples (2 isolates) were examined for invitro sensitivity to l4 antimicrobial agents and for presence of plasmid DNA. Randomly amplified polymorphic DNA (RAPD) analysis was used to type the isolates, using two arbitrary primers. All isolates were sensitive to chloramphenicol, kanamycin, carbenicillin, rifampicin, enrofloxacin, tetracycline and sulfamethoxazole trimethoprim. No plasmid was detected in all the isolates tested. RADP fingerprinting demonstrated genomic relationship between isolates, which provides an effective method to study the epidemiology of the isolates examined.

Item Type: Article
Uncontrolled Keywords: B)rkholderia pseudomalli, soil samples (2 isolates), l4 antimicrobial agent, RADP fingerprinting.
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation
Faculties, Institutes, Centres > Institute of Biodiversity and Environmental Conservation
Depositing User: Lihan
Date Deposited: 25 Aug 2022 01:30
Last Modified: 25 Aug 2022 01:30
URI: http://ir.unimas.my/id/eprint/39303

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